silica gel 60 Search Results


silica gel  (MACHEREY NAGEL)
96
MACHEREY NAGEL silica gel
Silica Gel, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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silica gel 60  (MACHEREY NAGEL)
95
MACHEREY NAGEL silica gel 60
Silica Gel 60, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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silica  (MACHEREY NAGEL)
93
MACHEREY NAGEL silica
Silica, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polygoprep 60 50 c18 silica gel  (MACHEREY NAGEL)
88
MACHEREY NAGEL polygoprep 60 50 c18 silica gel
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Polygoprep 60 50 C18 Silica Gel, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polygoprep 60 80 c18  (MACHEREY NAGEL)
92
MACHEREY NAGEL polygoprep 60 80 c18
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Polygoprep 60 80 C18, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polygoprep 60 30 nh2 lc packing material  (MACHEREY NAGEL)
91
MACHEREY NAGEL polygoprep 60 30 nh2 lc packing material
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Polygoprep 60 30 Nh2 Lc Packing Material, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tlc silica gel 60 aluminum sheet 1.05553.0001  (Merck KGaA)
90
Merck KGaA tlc silica gel 60 aluminum sheet 1.05553.0001
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Tlc Silica Gel 60 Aluminum Sheet 1.05553.0001, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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silica gel 60  (EM Science Inc)
90
EM Science Inc silica gel 60
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Silica Gel 60, supplied by EM Science Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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silica gel 60 (0.06-0.2)  (Carl Roth GmbH)
90
Carl Roth GmbH silica gel 60 (0.06-0.2)
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Silica Gel 60 (0.06 0.2), supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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silica gel 60 (230–400 mesh astm e. qingdao)  (Tsingtao Brewery Co)
90
Tsingtao Brewery Co silica gel 60 (230–400 mesh astm e. qingdao)
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Silica Gel 60 (230–400 Mesh Astm E. Qingdao), supplied by Tsingtao Brewery Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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silica gel 60-200 70-230 mesh, 60å  (SiliCycle)
90
SiliCycle silica gel 60-200 70-230 mesh, 60å
Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP <t>C18</t> HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.
Silica Gel 60 200 70 230 Mesh, 60å, supplied by SiliCycle, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP C18 HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.

Journal: Nucleic Acids Research

Article Title: Differential redox sensitivity of tRNA dihydrouridylation

doi: 10.1093/nar/gkae964

Figure Lengend Snippet: Effect of paraquat treatment on bacterial growth and dihydrouridine modification levels in E. coli . ( A ) Redox cycle of paraquat with oxygen. The scheme is adapted from Gray et al. . ( B ) Growth behaviour of E. coli cultures after paraquat treatment with different concentrations. ( C ) Reduction of uridine to dihydrouridine catalysed by the dihydrouridine synthase (Dus) requiring NADPH as redox equivalent. ( D ) LC–MS/MS analysis of dihydrouridine levels in total tRNA isolated from wild-type (WT, blue) E. coli cultures treated with paraquat (0, 0.1 and 0.3 mM). tRNA isolated from a knockout strain of all three dihydrouridine synthases ( ΔdusA ,B , C ) served as a negative control. The signals were normalized to the respective ultraviolet (UV) signal of adenosine and related to the signal of tRNA isolated from untreated WT culture. ( E ) Chromatographic separation of dihydrouridine (D, blue) in comparison to the main nucleosides cytidine (C), uridine (U), guanosine (G) and adenosine (A) (grey) on an RP C18 HPLC column. Dihydrouridine was detected by mass spectrometry, while the signals of the main nucleosides and the signal of the deaminase inhibitor pentostatin (PS) were detected by UV spectrometry at a wavelength of 254 nm.

Article Snippet: The flow-through was incubated on 0.1 g POLYGOPREP 60–50 C18 silica gel (Macherey-Nagel) before being filtered through 3 kDa cut-off filters (Vivaspin, Merck) and subjected to LC–MS/MS measurement.

Techniques: Modification, Liquid Chromatography with Mass Spectroscopy, Isolation, Knock-Out, Negative Control, Comparison, Mass Spectrometry